A reverse phase high performance liquid chromatography [RP-HPLC] method has been developed for the estimation of Nevirapine in bulk drug and pharmaceutical dosage forms. The quantification was carried out on Hypersil BDS column in isocratic mode, with mobile phase consisting of phosphate buffer and acetonitrile in the ratio of 55:45[v/v].The mobile phase was pumped at a rate of 1.0 ml/min and the detection was carried out at 214 nm and the linearity was found to be in the range of 10 to 60 μg/ml. The regression equation was found to be Y=105.58x + 1.1121 with correlation coefficient [r2] of 0.9998. The % recovery values were found to be in the range of 100.18-101.08%. The proposed method was validated for accuracy and precision. Statistical analysis proves that the method was found to be simple, precise, accurate, rapid and reproducible and can be used for the routine determination of Nevirapine in bulk drug and in pharmaceutical formulations.
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